Bisphenol A, Bisphenol F und Bisphenol S – Bestimmung von Bisphenol A, Bisphenol F und Bisphenol S in Urin mittels UPLC-ESI-MS/MS
Biomonitoring-Methode
Thomas Jäger1 (Methodenentwicklung)Michael Bader1 (Methodenentwicklung)
Katharina Sander2 (Methodenprüfung)
Katharina Blümlein2 (Methodenprüfung)
Thomas Göen3 (Methodenentwicklung; Leitung der Arbeitsgruppe „Analysen in biologischem Material“ der Ständigen Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft)
Andrea Hartwig4 (Vorsitz der Ständigen Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft)
MAK Commission5
1 BASF SE, Corporate Health Management, ESG/CB – H306, Carl-Bosch-Straße 38, 67056 Ludwigshafen, Deutschland
2 Fraunhofer Institut für Toxikologie und Experimentelle Medizin (ITEM), Arbeitsgruppe Bio- und Umweltanalytik, Nikolai-Fuchs-Straße 1, 30625 Hannover, Deutschland
3 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institut und Poliklinik für Arbeits-, Sozial- und Umweltmedizin, Henkestraße 9–11, 91054 Erlangen, Deutschland
4 Institut für Angewandte Biowissenschaften, Abteilung Lebensmittelchemie und Toxikologie, Karlsruher Institut für Technologie (KIT), Adenauerring 20a, Geb. 50.41, 76131 Karlsruhe, Deutschland
5 Ständige Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Deutschland
Abstract
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and verified the presented biomonitoring method. Bisphenol A (BPA), bisphenol F (BPF), and bisphenol S (BPS) are co-monomers for producing engineering plastics used in the automotive, food, and household sectors and are also used in paper chemicals and leather-tanning agents. Due to the wide range of applications, exposure to these bisphenols can occur both at the workplace and via the environment. The aim of this work was to develop a selective method for the determination of BPA, BPF, and BPS in human urine. This method has been comprehensively verified, and the reliability data have been confirmed by replication and validation of the procedure in a second, independent laboratory. The internal standards are added to the buffered urine samples, which are then subjected to enzymatic hydrolysis and processed via dispersive liquid-liquid microextraction (DLLME). The analytes are separated from matrix components by liquid chromatography and detected by tandem mass spectrometry using electrospray ionisation. Quantitative evaluation is carried out via external calibration in water. The good precision and accuracy data show that the method provides reliable and accurate measurement values. The method is selective and sensitive, and the quantitation limits of 0.25 μg/l, 0.10 μg/l, and 0.05 μg/l urine for BPA, BPF, and BPS, respectively, are sufficient to determine occupational as well as background exposure.
