2-Phenoxyethanol – Bestimmung von Phenoxyessigsäure und 4-Hydroxyphenoxyessigsäure in Urin mittels UPLC-ESI-MS/MS
Biomonitoring-Methode
Thomas Jäger1 (Methodenentwicklung)Michael Bader1 (Methodenentwicklung)
Laura Kuhlmann2 (Methodenprüfung)
Thomas Göen2 (Leitung der Arbeitsgruppe „Analysen in biologischem Material“ der Ständigen Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft; Methodenprüfung)
Andrea Hartwig3 (Vorsitz der Ständigen Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft)
MAK Commission4
1 BASF SE, Corporate Health Management, ESG/CB – H306, Carl-Bosch-Straße 38, 67056 Ludwigshafen, Deutschland
2 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institut und Poliklinik für Arbeits-, Sozial- und Umweltmedizin, Henkestraße 9–11, 91054 Erlangen, Deutschland
3 Institut für Angewandte Biowissenschaften, Abteilung Lebensmittelchemie und Toxikologie, Karlsruher Institut für Technologie (KIT), Adenauerring 20a, Geb. 50.41, 76131 Karlsruhe, Deutschland
4 Ständige Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Deutschland
Abstract
The working group “Analyses in Biological Materials” of the German Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and verified the presented method for the biomonitoring of exposure to 2-phenoxyethanol, an important biocide. The method described herein allows for the determination of the two main metabolites of 2-phenoxyethanol, specifically phenoxyacetic acid (PhAA) and 4-hydroxyphenoxyacetic acid (4-OH-PhAA), in urine. The urine samples are mixed with diluted formic acid and the internal standards. The analytes are separated from matrix components by liquid chromatography and subsequently analysed by tandem mass spectrometry using electrospray ionisation. Quantitative evaluation is carried out via external calibration in ultra-pure water. Good precision data with standard deviations below 7.19% for PhAA and below 8.07% for 4-OH-PhAA as well as good accuracy data with mean relative recoveries in the range of 98.7–109% for PhAA and of 92.6–107% for 4-OH-PhAA show that the method provides reliable and accurate analytical results. The method is both selective and sensitive, and the limits of quantitation of 8.2 μg PhAA/l urine and 7.6 μg 4-OH-PhAA/l urine are sufficient to determine occupational exposure as well as background exposure of the general population to 2-phenoxyethanol.
